NAD+ 100mg

Mechanism Studied in Research

NAD+ acts at the intersection of redox biology, energy regulation and DNA-damage signalling. As a hydride acceptor, it supports glycolysis, beta-oxidation and the TCA cycle, generating NADH that feeds oxidative phosphorylation through complex I of the electron transport chain.
The reduced and oxidised pools NAD+/NADH and NADP+/NADPH define cellular redox state and influence pathways from antioxidant defence to lipid synthesis. NAD+ is also consumed as a substrate by sirtuins, PARPs and CD38, linking its availability to deacetylation, DNA repair, calcium signalling and immune-cell metabolism.
Research has linked restoration of NAD+ pools to mitochondrial function, stress response and cellular ageing models. The 100mg vial supports defined in-vitro and preclinical work where investigators need controlled concentrations without committing to larger material volumes.

Research Applications

Pilot studies and method development. The 100mg vial format supports laboratories establishing new NAD+ assays, validating cell-culture models or training new researchers in sirtuin and bioenergetics workflows.
Sirtuin and redox research. NAD+ is used in enzyme assays, deacetylase readouts and redox-state studies where investigators monitor NAD+/NADH balance, protein acetylation and mitochondrial activity.
Mitochondrial bioenergetics research. NAD+ is commonly used in respirometry studies, Seahorse assays and oxidative phosphorylation research where cellular energy metabolism is measured under defined conditions.
Cellular ageing and DNA-repair studies. NAD+ is investigated in PARP biology, oxidative stress models and cellular senescence research, particularly where DNA-damage signalling and repair capacity are central endpoints.

Reconstitution Reference

NAD+ 100mg is supplied as a sterile lyophilised powder under vacuum seal. It is soluble in sterile water for injection and bacteriostatic water containing 0.9% benzyl alcohol, both commonly used as reconstitution solvents in research stocks.
A practical example is the addition of 2ml of bacteriostatic water, producing a 50mg/ml stock solution. The diluent should be introduced slowly down the inner wall of the vial to minimise foaming and mechanical disruption.
The solution should dissolve fully and appear clear before use. NAD+ solutions are sensitive to heat and alkaline pH, so keep working stocks cold and use them promptly.

Storage and Handling

Lyophilised NAD+ should be stored at -20°C in the original vial, protected from light and moisture. Short transit at 2–8°C is acceptable, and sealed vials should be returned to frozen storage after receipt.
Once reconstituted, NAD+ working solutions should be refrigerated at 2–8°C and used promptly according to laboratory protocol. Repeated freeze-thaw cycles should be avoided.
Researchers should aliquot working solutions where appropriate and document reconstitution date, diluent volume and final concentration. Any solution showing discoloration or particulate matter should be discarded.

References